Effective Gene Delivery And Its Effects On Hiv - 987 Words

There has been countless number of efforts to develop an effective gene transfer approach to treat HIV-1 infections globally. Many transgenes have been identified to inhibit in vitro HIV-1 infections. As drug resistant HIV-1 is increasingly common even with patients receiving HAART, designing drug treatment methods have been challenging. Therefore, investigation into new therapeutic approaches should continue. Gene delivery plays an important supporting role developing potential therapies directed towards HIV-1 infections. The goals of the anti-HIV-1 gene therapy are to deliver transgenes to directly susceptible cells, Immunize against HIV-1 antigens, inhibit HIV-1 proliferation in target organs, and deliver to Hematopoietic Progenitor Cells. Delivering transgenes to susceptible cells will make them resistant to HIV-1 infections and inhibit viral replication. When CCR5 a major coreceptor for HIV was disrupted in the â€Å"Berlin Patient† led to the revival of gene therapy . The naturally occurring CCR5-Δ32 mutation that results in a frameshift mutation dislocates the CCR5 expression on the cell surface. The â€Å"Berlin patient† who was HIV+ patient with lymphoma was transplanted with bone marrow from a donor with CCR5-Δ32 mutation and became ‘cured’ of HIV. But as only a small general population with CCR5-Δ32 mutation can be identified alternative methods must be identified for gene therapy. Though gene therapies for HIV-1 infections have been challenging recent work must beShow MoreRelatedThat Target The Gene Codes For A Protein Found On The Surface Of White Blood Cells1671 Words   |  7 PagesZFNs that Target the Gene CCR5 The CCR5 gene codes for a protein found on the surface of white blood cells that acts as a receptor for chemokines. The HIV virus, strain R5-tropic virus, initially uses the CCR5 chemokine receptor to attach to the CD4+ helper T-cells. The Berlin patient showed how a CCR5-negative hematopoietic stem/ progenitor cells (HSC) from a CCR5 ∆ 32 donor can be used to generate HIV-1 resistant CD4+ helper T-cells.3 Mice models using in vivo studies have also shown ZFNs to beRead MoreUseful Medicinal Compounds Derived from Animals632 Words   |  3 Pagesimmune system can recognize. Finally, immune response will be triggered. Tumor cell can be killed by B-cell or Phagocyte. Over a hundred years ago, target drug idea has been proposed. If a drug can be delivery to the target site precisely, the adverse effect of the drug can be minimized. â€Å"Delivery the drug to the target site† became a challenge the scientist. In the 1970s, B-cell has been discovered and played essential role (Biotech Resources, 1995-7). Anyways, as the technology, antibody cannotRead MoreFoamy Virus Case Study1329 Words   |  6 Pages2017) The foamy viral primer, central polypurine tract(cPPT) produced gap in double stranded viral DNA of 144 to 731 bp. The 3 and 5’ terminus of the cleavage is located between 6272-6274 bp and 465 bp range respectively. Mutation of fourth cPPT and HIV-1 virus resulted in a substantial attenuation of foamy virion indicates the indispensability of cPPT for viral DNA synthesis. (Chen et al., 2017) Cytotoxicity and multiple transduction rounds of current viral vectors necessitate the foamy virus vectorRead MoreThe Usefulness Of Viral Vectors1223 Words   |  5 PagesVoltage-gated sodium channels initiate the development action potentials in neurons; mutations of genes encoding these channels are responsible for genetic epilepsy syndromes with varying degrees of severity1. Dravet syndrome, or severe myoclonic epilepsy of infancy (SMEI), is a complex and severe form of childhood epilepsy, caused by a nonsense mutation in the sodium voltage-gated channel alpha subunit 1 (SCN1a) gene. Severe loss of function mutation in Nav1.1 causes SMEI, which involves intractable epilepsyRead MoreNanoparticles : HIV And The Human Immunodeficiency Virus1085 Words   |  5 Pagesin targeted drug delivery while minimizing toxicity. Gold is used as the basis for nanoparticles because it has a relatively high stability of ligand binding along with its decreased stability with other cells. Gold nan oparticles (AuNPs) also have a high concentration of glutathione, which is an antioxidant that prevents damage to cells from things like free radicals and peroxides. We are using AuNPs to carry drugs to cells infected with the Human Immunodeficiency Virus (HIV). HIV is a virus in theRead MoreGene Silencing : Current Approaches And Relevance For Drug Discovery2632 Words   |  11 PagesTITLE: Gene Silencing – Current Approaches and Relevance for Drug Discovery NEW TITLE: OUTLINE: Abstract Gene silencing is an evolving field that holds great promise for personalized drug discovery. The ability to interfere with mutated genes gives rise to the possibility of shutting down disease causing mechanisms and the advent of personalized drugs for specific diseases. The prospect of easily silencing genes in the lab for functional genomic studies has not fully crossed over into theRead MoreGlucagon Like Peptide-1 Essay791 Words   |  4 Pageseating. GLP-1 has many beneficial effects in diabetes including (but not limited to) activation of somatostatin secretion from delta cells, inhibition of glucagon release from alpha cells, reduced gastric emptying, and food intake resulting in weight loss in the long run. Unfortunately, the incretin response to glucose is reduced in type 2 diabetes (T2DM) accompanied by a moderate degree of GLP-1 hypo-secretion. To compensate the reduced incretin effect, we generated an HIV-based lentiviral vector to deliverRead MoreGene Therapy And Its Effects1619 Words   |  7 Pagespatients are treated. Recently, gene therapy has been propelled into the spotlight with the most recent successful clinical trial in which scientists reprogrammed ordinary cardiac cells in pigs to mirror the function of the sinoatrial node, thereby inducing a steady heartbeat. In the last few decades since its inception, gene therapy has rapidly advanced; there have been remarkable strides in its methods and an increase in the range of targetable diseases. Gene therapy is the modification of theRead MoreCase Study : Rna Interference : What Advances Have Been Made From The Last Decade?1435 Words   |  6 Pages................................................................ 8 Delivery ...................................................................................................................................................................8 Stability ...................................................................................................................................................................9 Off-Target Effect...................................................................Read MoreEssay about Priotities of Gene Therapy2802 Words   |  12 PagesPriotities of Gene Therapy Gene therapy is a relatively new area of medicine that attempts to apply recent advances in molecular biology, genetics and biotechnology to the treatment of human diseases. Gene therapy uses a set of approaches to the treatment of human disease based on the transfer of genetic material (DNA) into an individual. Gene delivery can be achieved either by direct administration of gene-containing viruses or DNA to blood or tissues, or indirectly through the introduction